Anton Glieder

Xavier García-Ortega

Karin Kovar

Lukas Rieder

This volume provides readers with a useful and comprehensive resource of laboratory protocols for working with Pichia pastoris. The chapters in this book are organized into seven parts: introduction, bioinformatics, cell and systems biology, gene expression cassettes and vectors, Pichia pastoris expression strains, upstream bioprocessing, and downstream processing and product analysis. This book covers basic and newer laboratory methods, starting from the gene and genetic construct to mature (yet reasonable) amounts of a recombinant product being produced, purified, and characterized. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Pichia pastoris: Methods and Protocols is a valuable resource for both new and experienced researchers who want to learn more about this important and developing field.

Methods and Protocols

Pichia pastoris

Michael Sauer

Christian P. Kubicek

Diethard Mattanovich

Birgit Wiltschi

This book provides a comprehensive, up-to-date overview of the opportunities and challenges of the complex field of synthetic biology, which combines various scientific disciplines. The emerging field of synthetic biology employs biotechnological approaches to recreate and enhance basic biological structures, intracellular processes and whole organisms. The book addresses a broad range of topics, including redesigning complex metabolic pathways, DNA/RNA and protein engineering, as well as novel synthetic biomaterials. It discusses both “bottom up” and “top down” approaches and presents the latest genome engineering tools with predictions about how these could change our way of thinking and working. Since the use of synthetic biology raises a number of ethical questions, a chapter is devoted to public awareness and risk management.The book is of interest to scientists from both academia and industry, as well as PhD students and postdocs working in the field

Synthetic Biology

Anton Glieder

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