EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD.

William G. Kavanagh

Dennis M. Hinton

David E. Lenz

Ludwig A. Sternberger

Expulsion of hemin from horseradish peroxidase at pH 2.1 is accompained by aggregation of the protein. The process is completely reversible after short periods in acid and is partially reversible after long periods (48 hours). On gel filtration of peroxidase immediately after acidification, hemin and the protein elute together. Apoperoxidase (pH 2.1) elutes much later (about the same elution volume as neutral enzyme) than peroxidase (pH 2.1) indicating a hemin requirement for the aggregation. Analytical sedimentation velocity and sucrose density gradient sedimentation of peroxidase immediately after acidification reveal a 7.8S component with both 400-nm and 280-nm absorbance and a 4S component with 280-nm abosrbance. Sedimentation velocity studies of hemin in the presence of bovine serum albumin or rabbit immunoglobulin G reveal hemin aggregation without hemin precipitation or protein aggregation. A molecular weight of about 80,000 for the peroxidase-hemin aggregate has been determined by membrane osmometry.

Role of Hemin in the Acid Aggregation of Horseradish Peroxidase

Frederick R. Sidell

The case histories of five patients accidentally exposed to anticholinesterase compounds (sarin or soman) are presented. The circulating cholinesterase of one patient severely exposed to soman was refractory to oxime reactivation; this patient also had psychological sequelae which were ameliorated by scopolamine. In three patients who had mild manifestations of sarin intoxication the initial rate of recovery of plasma cholinesterase paralleled the recovery of their ability for adaptation to the dark, or loss of miosis. An anticholinergic compound with high central nervous system affinity, such as scopolamine, should be tried further when psychological morbidity follows anticholinesterase compound intoxication. (Author).

Sarin and Soman: Observations on Accidental Exposures

Woodrow W. Reaves

Melvin N. Gerber

Stanley M. White

The object of the study described in the report was to develop a pyrotechnic mixture which, when functioned in an E49 unit, would produce a white smoke to simulate the CS cloud thermally produced from the same munition. Intimate mixtures of 2,4-dehydroxy benzophenone and various pyrotechnic blends pressed into E49 units have shown promise in that they have given clouds which resemble CS clouds thermally generated from E49 units. (Author).

A White Smoke to Simulate CS Smoke

EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD.

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