Lynda Williams

Gerard Morel

Annie Cavalier

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization

In Situ Hybridization in Electron Microscopy

A multiplicity of methods for visualization are described in Visualization of Receptors. This book of techniques covers immunocytology, radioautography, in situ hybridization, plasmon resonance, RT in vitro PCR, and X-ray crystal structure analysis. Lecturers, researchers, practitioners, technicians, and students will find all the principles and protocols they require and the means of implementing them. Topics discussed include localization of ligands (in vitro or in vivo), visualization of immunoreactivities of ligand and receptor proteins, detection and quantification of mRNA expression, amplification of signals, and determination of 3-D structure. Details of protocols with illustrations of results and commentaries are provided throughout the book.

Visualization of Receptors

Hybridization Techniques for Electron Microscopy examines the use of in situ hybridization techniques, including an overview of current perspectives and future developments. The book features in situ methods for fluorescence probes and confocal scanning microscopes. Three in situ hybridization methods for electron microscopes are analyzed: the non-embedded tissue method using ultrathin frozen sections, pre-embedded method, and post-embedded method using material embedded in hydrophilic resin. Positive and negative features are discussed, and clear instructions regarding implementation of techniques are provided. Particular aspects of the techniques are examined in detail, such as preparation of tissue, pretreatment, hybridization procedures, revelation (autoradiography and immunocytology) and checking procedures, in addition to the illustration, interpretation, and discussion of methods and results. The main applications described include virus detection, chromosomal gene mapping, detection of ribosomic nucleic acid, and detection of messenger RNA in animals and plants. Hybridization Techniques for Electron Microscopy is an excellent reference for cytologists, cell biologists, histochemists, cytochemists, molecular endocrinologists, and neuroendocrinologists.

Hybridization Techniques for Electron Microscopy

In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used by molecular biologists, pathologists, geneticists, and biochemists. It will also appeal to research scientists who are interested in visualizing methods for nucleic acids and proteins. The book features a two-column layout for protocols/principles, simplifies the techniques and offers many schemes and tables to help the reader choose the best procedure.

In Situ Hybridization in Light Microscopy

Alexandre Dolgui

Carlos Eduardo Pereira

Information Control Problems in Manufacturing 2006 contains the Proceedings of the 12th IFAC Symposium on Information Control Problems in Manufacturing (INCOM'2006). This symposium took place in Saint Etienne, France, on May 17-19 2006. INCOM is a tri-annual event of symposia series organized by IFAC and it is promoted by the IFAC Technical Committee on Manufacturing Plant Control. The purpose of the symposium INCOM'2006 was to offer a forum to present the state-of-the-art in international research and development work, with special emphasis on the applications of optimisation methods, automation and IT technologies in the control of manufacturing plants and the entire supply chain within the enterprise. The symposium stressed the scientific challenges and issues, covering the whole product and processes life cycle, from the design through the manufacturing and maintenance, to the distribution and service. INCOM'2006 Technical Program also included a special event on Innovative Engineering Techniques in Healthcare Delivery. The application of engineering and IT methods in medicine is a rapidly growing field with many opportunities for innovation. The Proceedings are composed of 3 volumes: Volume 1 - Information Systems, Control & Interoperability Volume 2 - Industrial Engineering Volume 3 - Operational Research * 3-volume set, containing 362 carefully reviewed and selected papers * presenting the state-of-the-art in international research and development in Information Control problems in Manufacturing

Gerard Morel

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