Ajit Varma

Gopi K. Podila

This book provides a survey on the most striking and successful appraoches to produce biogenic nanodevices with emphasis onthe use of microorganisms for production. Chapters are dedicated to the biotechnological production of tailor-made biopoler-based self-assembled nanostructures such as biopolyester inclusions, cyanophycin inclusions and alginates, which can be processed into nanoparticles. Other chapters summarize recent developments in protein and DNA-based nanodevice production. The aim of this book is to demonstrate the diversity of biological nanostructures, the implied design space, and the enormous potential for applications in medicine and technology. Potential applications range from drug delivery to biocomputing.

Biotechnological Applications of Microbes

Peter B. Kaufman

Leland J. Cseke

Chung-Jui Tsai

Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Gopi K. Podila

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