Shri Mohan Jain

Maurizio Lambardi

Elif Aylin Ozudogru

Micropropagation is a reliable technology applied commercially worldwide for large-scale plant multiplication, germplasm conservation, pathogen elimination, genetic manipulations and supply of selected plants. In Protocols for Micropropagation of Selected Economically-Important Horticultural Plants, well recognised researchers in the field compile step-wise protocols for rapid plant multiplication of economically-important horticultural species. The book contains 35 chapters, divided into four major sections. The first three sections (Section A, B and C) contain 29 micropropagation protocols of selected fruit and nut species, indoor and outdoor ornamental plants, cut flowers, and vegetables. In addition to the detailed protocols of in vitro shoot initiation, proliferation, root induction and acclimatization, chapters also include detailed information on medium preparation, explant selection and preparation. The six chapters of Section D cover specific reviews on pivotal topics, such as in vitro rejuvenation, synthetic seed technology, thermotherapy and meristem culture in banana, genetic transformation of pineapple, flower color somaclonal variation in torenia, and cryotherapy of horticultural crops. Moreover, as a part of the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topic, lists of necessary materials, notes, and illustrative photos. Comprehensive and well-written, Protocols for Micropropagation of Selected Economically-Important Horticultural Plants offers a useful resource for horticulturists, researchers, commercial companies, plant propagators, biotechnologists and students interested in micropropagation.

Protocols for Micropropagation of Selected Economically-Important Horticultural Plants

Maria Antonietta Germanà

This volume presents an overview of recent advances, innovative applications, and future prospects of in vitro embryogenesis in higher plants. The book's chapters are divided into five parts: Part I contains reviews on general topics (microspore; zygotic and somatic embryogenesis; in vitro and in vivo asexual embryogenesis; advances on the genetic, physiological, and proteomic knowledge of somatic embryo formation; role of apoptosis and mitochondria in somatic embryogenesis; and innovation in the use of bioreactors). The remaining four parts discuss step-wise protocols on somatic embryogenesis in selected horticultural plants (Part II); forest trees (Part III); gametic embryogenesis (Part IV); and pivotal topics, such as the detection of epigenetics modifications during microspore embryogenesis, the in vitro embryogenesis and plant regenerations from isolated zygotes, the synthetics seed production, the induction and maturation of somatic embryos, and the cryostorage of embryogenic cultures (Part V). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, In Vitro Embryogenesis in Higher Plants, is a useful source of information and ideas for plant tissue culturists, cell biologists, embryologists, horticulturists, and operators of commercial nurseries. This book will introduce the fascinating work in in vitro embryogenesis in higher plants to students and young scientists.

Maurizio Lambardi

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