Prem Jose Vazhacharickal

Noby Mathew

Elizabeth Cherian

Scientific Study from the year 2017 in the subject Biology - Botany, grade: 1.5, Mar Augusthinose College, language: English, abstract: In this study, the callus initiation and rhizogenesis of Justicia gendarussa from nodal and leaf explants were established in Murashige and Skoog medium supplemented with different hormonal concentrations and also optimization of callus initiation at hormonal concentrations. As a result the callus was initiated on MS medium supplemented with different hormones. The higher concentration of NAA and BA induced callus from nodal and leaf explants. The higher concentrations of 2, 4 – D and kinetin induced callus on nodal explants. Lower concentration of 2, 4 – D and kinetin induce callus on leaf explants. The combination of NAA and kinetin induce rhizogenesis from nodal explants. The antimicrobial activity of callus was evaluated by well diffusion method. Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilus, Staphylococcus aureus, Klebsiella pneumonia were used as test organisms. The results showed that Justicia gendarussa have antimicrobial activity on Escherichia coli, Klebsiella pneumonia, and Staphylococcus aureus. Keywords: Justicia gendarussa, callus initiation, direct organogenesis, antimicrobial assay.

The Uses and Advantages of an Exotic Medicinal Plant in Kerala

Optimization of Media Formulations for Callus Induction and direct Organogenesis in Justicia Gendrussa Burm Fil

Lakshmi B Nair

A simple and reproducible protocol for in vitro callus induction from explants of three endemic plants (Cyclea peltata, Naregamia alata and Kaempferia galanga Linn.) have been developed. Explants collected from the field grown plants were cultured on MS medium supplemented with different concentration/combination (s) of phytohormones. During the study period we evaluated the effect of different growth regulators in callus induction and its morphological analysis of the targeted plants. To optimize the callus induction of three different targeted explants were cultured on different concentration phytohormones. Among which the system include 2,4-D has the most efficient effect on the three experimental plants, Five different concentration taken for three explants, among that Cyclea peltata and Kaempferia galangal Linn. has the highest potential to induce callusing at 2 mg/L of 2,4 -D. In this study we found that there was no effect on callusing of the targeted plants was MS medium containing combination of auxin and cytokinin for callusing.

Efficient in vitro callus induction protocol for three endemic medicinal plants (Cyclea peltata, Naegamia alata and Kaempferia galangal Linn.) in Kerala

Athira T Rameshan

Caesalpinia pulcherrima flowers have excellent antibacterial property. The present study aims to evaluate the antibacterial property Caesalpinia pulcherrima flower extract against clinically isolated eye infection causing Staphylococcus auerus. The (MIC) minimum inhibitory concentration and (MBC) minimum bactericidal concentration was found to be 200 µg/ml and 400 µg/ml. Antioxidant activity, spectroscopic and phytochemical analysis of the extract was also studied. This study concludes that Caesalpinia pulcherrima can be used as a potential natural remedy to treat eye infection.

Noby Mathew

Books

Your next favorite book is out there