Shabir Hussain Wani

Ali Raza

Karansher Singh Sandhu

Sunil S. Gangurde

Breaking the myth: Breeding for stress tolerance, grain yield, and quality traits simultaneously by diversifying the narrow genetic base

Satbir Singh Gosal

Plant improvement has shifted its focus from yield, quality and disease resistance to factors that will enhance commercial export, such as early maturity, shelf life and better processing quality. Conventional plant breeding methods aiming at the improvement of a self-pollinating crop, such as wheat, usually take 10-12 years to develop and release of the new variety. During the past 10 years, significant advances have been made and accelerated methods have been developed for precision breeding and early release of crop varieties. This work summarizes concepts dealing with germplasm enhancement and development of improved varieties based on innovative methodologies that include doubled haploidy, marker assisted selection, marker assisted background selection, genetic mapping, genomic selection, high-throughput genotyping, high-throughput phenotyping, mutation breeding, reverse breeding, transgenic breeding, shuttle breeding, speed breeding, low cost high-throughput field phenotyping, etc. It is an important reference with special focus on accelerated development of improved crop varieties.

Food Legumes

Accelerated Plant Breeding, Volume 3

Gyanendra Pratap Singh

Vennampally Nataraj

With the erratic changes in climate, crop plants are facing many forms of biotic stresses. When plants are under stress, among several gene families, regulatory genes play a vital role in signal transduction in modulating the expression of genes underpinning several defense pathways and targeting regulatory proteins (viz, transcription factors (TFs)) can be the alternative. Transcription factors directly regulate the downstream R genes and are excellent candidates for disease resistance breeding. Till date, numerous transcription factors have been identified and characterized structurally and functionally. Of them, TF families such as WRKY, NAC, Whirly, Apetala2 (AP2), ethylene responsive elements (ERF) etc. are found to be associated with transcriptional reprogramming of plant defense response. These TFs are responsive to the pathogen’s PAMPs/DAMPs - host’s PRR protein interactions and specifically binds to the cis-elements of defense genes and regulate their expression. With this background, realizing the importance of TFs in resistance breeding, this book discusses the recent research and developments in this field for various crops.

Transcription Factors for Biotic Stress Tolerance in Plants

P.E. Rajasekharan

Medicinal plants are globally valuable sources of herbal products. Plant-based remedies have been used for centuries and have had no alternative in the western medicine repertoire, while others and their bioactive derivatives are in high demand and have been the central focus of biomedical research. As Medicinal plants move from fringe to mainstream with a greater number of individuals seeking treatments free of side effects, considerable attention has been paid to utilize plant-based products for the prevention and cure of human diseases. An unintended consequence of this increased demand, however, is that the existence of many medicinal plants is now threatened, due to their small population size, narrow distribution area, habitat specificity, and destructive mode of harvesting. In addition, climate change, habitat loss and genetic drift have further endangered these unique species. Although extensive research has been carried out on medicinal and aromatic plants, there is relatively little information available on their global distribution patterns, conservation and the associated laws prevailing. This book reviews the current status of threatened medicinal plants in light of increased surge in the demand for herbal medicine. It brings together chapters on both wild (non-cultivated) and domestic (cultivated) species having therapeutic values. Thematically, conventional and contemporary approaches to conservation of such threatened medicinal plants with commercial feasibility are presented. The topics of interest include, but not limited to, biotechnology, sustainable development, in situ and ex situ conservation, and even the relevance of IPR on threatened medicinal plants. We believe this book is useful to horticulturists, botanists, policy makers, conservationists, NGOs and researchers in the academia and the industry sectors.

Conservation and Utilization of Threatened Medicinal Plants

Human population is escalating at an enormous pace and is estimated to reach 9.7 billion by 2050. As a result, there will be an increase in demand for agricultural production by 60–110% between the years 2005 and 2050 at the global level; the number will be even more drastic in the developing world. Pathogens, animals, and weeds are altogether responsible for between 20 to 40 % of global agricultural productivity decrease. As such, managing disease development in plants continues to be a major strategy to ensure adequate food supply for the world. Accordingly, both the public and private sectors are moving to harness the tools and paradigms that promise resistance against pests and diseases. While the next generation of disease resistance research is progressing, maximum disease resistance traits are expected to be polygenic in nature and controlled by selective genes positioned at putative quantitative trait loci (QTLs). It has also been realized that sources of resistance are generally found in wild relatives or cultivars of lesser agronomic significance. However, introgression of disease resistance traits into commercial crop varieties typically involves many generations of backcrossing to transmit a promising genotype. Molecular marker-assisted breeding (MAB) has been found to facilitate the pre-selection of traits even prior to their expression. To date, researchers have utilized disease resistance genes (R-genes) in different crops including cereals, pulses, and oilseeds and other economically important plants, to improve productivity. Interestingly, comparison of different R genes that empower plants to resist an array of pathogens has led to the realization that the proteins encoded by these genes have numerous features in common. The above observation therefore suggests that plants may have co-evolved signal transduction pathways to adopt resistance against a wide range of divergent pathogens. A better understanding of the molecular mechanisms necessary for pathogen identification and a thorough dissection of the cellular responses to biotic stresses will certainly open new vistas for sustainable crop disease management. This book summarizes the recent advances in molecular and genetic techniques that have been successfully applied to impart disease resistance for plants and crops. It integrates the contributions from plant scientists targeting disease resistance mechanisms using molecular, genetic, and genomic approaches. This collection therefore serves as a reference source for scientists, academicians and post graduate students interested in or are actively engaged in dissecting disease resistance in plants using advanced genetic tools.

Molecular, Genetic and Genomic Perspectives

Disease Resistance in Crop Plants

Mukesh Jain

Advances in molecular biology and genome research in the form of molecular breeding and genetic engineering put forward innovative prospects for improving productivity of many pulses crops. Pathways have been discovered, which include regulatory elements that modulate stress responses (e.g., transcription factors and protein kinases) and functional genes, which guard the cells (e.g., enzymes for generating protective metabolites and proteins). In addition, numerous quantitative trait loci (QTLs) associated with elevated stress tolerance have been cloned, resulting in the detection of critical genes for stress tolerance. Together these networks can be used to enhance stress tolerance in pulses. This book summarizes recent advances in pulse research for increasing productivity, improving biotic and abiotic stress tolerance, and enhancing nutritional quality.

Physiological, Molecular and Genetic Perspectives

Pulse Improvement

Venura Herath

Cold stress is one of the prevalent environmental stresses affecting crop productivity, particularly in temperate regions. Numerous plant types of tropical or subtropical origin are injured or killed by non-freezing low temperature, and display a range of symptoms of chilling injury such as chlorosis, necrosis, or growth retardation. In contrast, chilling tolerant species thrive well at such temperatures. To thrive under cold stress conditions, plants have evolved complex mechanisms to identify peripheral signals that allow them to counter varying environmental conditions. These mechanisms include stress perception, signal transduction, transcriptional activation of stress-responsive target genes, and synthesis of stress-related proteins and other molecules, which help plants to strive through adverse environmental conditions. Conventional breeding methods have met with limited success in improving the cold tolerance of important crop plants through inter-specific or inter-generic hybridization. A better understanding of physiological, biochemical and molecular responses and tolerance mechanisms, and discovery of novel stress-responsive pathways and genes may contribute to efficient engineering strategies that enhance cold stress tolerance. It is therefore imperative to accelerate the efforts to unravel the biochemical, physiological and molecular mechanisms underlying cold stress tolerance in plants. Through this new book, we intend to integrate the contributions from plant scientists targeting cold stress tolerance mechanisms using physiological, biochemical, molecular, structural and systems biology approaches. It is hoped that this collection will serve as a reference source for those who are interested in or are actively engaged in cold stress research.

Cold Tolerance in Plants

Lam-Son Phan Tran

Mohammad Anwar Hossain

Soumen Bhattacharjee

David J Burritt

Drought is one of the most severe constraints to crop productivity worldwide, and thus it has become a major concern for global food security. Due to an increasing world population, droughts could lead to serious food shortages by 2050. The situation may worsen due to predicated climatic changes that may increase the frequency, duration and severity of droughts. Hence, there is an urgent need to improve our understanding of the complex mechanisms associated with drought tolerance and to develop modern crop varieties that are more resilient to drought. Identification of the genes responsible for drought tolerance in plants will contribute to our understanding of the molecular mechanisms that could enable crop plants to respond to drought. The discovery of novel drought related genes, the analysis of their expression patterns in response to drought, and determination of the functions these genes play in drought adaptation will provide a base to develop effective strategies to enhance the drought tolerance of crop plants. Plant breeding efforts to increase crop yields in dry environments have been slow to date mainly due to our poor understanding of the molecular and genetic mechanisms involved in how plants respond to drought. In addition, when it comes to combining favourable alleles, there are practical obstacles to developing superior high yielding genotypes fit for drought prone environments. Drought Tolerance in Plants, Vol 2: Molecular and Genetic Perspectives combines novel topical findings, regarding the major molecular and genetic events associated with drought tolerance, with contemporary crop improvement approaches. This volume is unique as it makes available for its readers not only extensive reports of existing facts and data, but also practical knowledge and overviews of state-of-the-art technologies, across the biological fields, from plant breeding using classical and molecular genetic information, to the modern omic technologies, that are now being used in drought tolerance research to breed drought-related traits into modern crop varieties. This book is useful for teachers and researchers in the fields of plant breeding, molecular biology and biotechnology.

Molecular and Genetic Perspectives

Drought Stress Tolerance in Plants, Vol 2

This edited volume summarizes the recent advancements made in plant science including molecular biology and genome editing , particularly in the development of novel pathways tolerant to climate change-induced stresses such as drought, extreme temperatures, cold, salinity, flooding, etc. These stresses are liable for decrease in yields in many crop plants at global level. Till date conventional plant breeding approaches have resulted in significant improvement of crop plants for producing higher yields during adverse climatic conditions. However, the pace of improvement through conventional plant breeding needs to be accelerated in keeping with the growing demand of food and increasing human populationl, particularly in developing world. This book serves as a comprehensive reference material for researchers, teachers, and students involved in climate change-related abiotic stress tolerance studies in plants.

Recent Approaches in Omics for Plant Resilience to Climate Change

Ajay Kumar Thakur

Yasin Jeshima Khan

Global population is mounting at an alarming stride to surpass 9.3 billion by 2050, whereas simultaneously the agricultural productivity is gravely affected by climate changes resulting in increased biotic and abiotic stresses. The genus Brassica belongs to the mustard family whose members are known as cruciferous vegetables, cabbages or mustard plants. Rapeseed-mustard is world’s third most important source of edible oil after soybean and oil palm. It has worldwide acceptance owing to its rare combination of health promoting factors. It has very low levels of saturated fatty acids which make it the healthiest edible oil that is commonly available. Apart from this, it is rich in antioxidants by virtue of tocopherols and phytosterols presence in the oil. The high omega 3 content reduces the risk of atherosclerosis/heart attack. Conventional breeding methods have met with limited success in Brassica because yield and stress resilience are polygenic traits and are greatly influenced by environment. Therefore, it is imperative to accelerate the efforts to unravel the biochemical, physiological and molecular mechanisms underlying yield, quality and tolerance towards biotic and abiotic stresses in Brassica. To exploit its fullest potential, systematic efforts are needed to unlock the genetic information for new germplasms that tolerate initial and terminal state heat coupled with moisture stress. For instance, wild relatives may be exploited in developing introgressed and resynthesized lines with desirable attributes. Exploitation of heterosis is another important area which can be achieved by introducing transgenics to raise stable CMS lines. Doubled haploid breeding and marker assisted selection should be employed along with conventional breeding. Breeding programmes aim at enhancing resource use efficiency, especially nutrient and water as well as adoption to aberrant environmental changes should also be considered. Biotechnological interventions are essential for altering the biosynthetic pathways for developing high oleic and low linolenic lines. Accordingly, tools such as microspore and ovule culture, embryo rescue, isolation of trait specific genes especially for aphid, Sclerotinia and alternaria blight resistance, etc. along with identification of potential lines based on genetic diversity can assist ongoing breeding programmes. In this book, we highlight the recent molecular, genetic and genomic interventions made to achieve crop improvement in terms of yield increase, quality and stress tolerance in Brassica, with a special emphasis in Rapeseed-mustard.

Molecular, Genetics and Genomic Perspectives

Brassica Improvement

Jauhar Ali

This book is open access under a CC BY 4.0 license. By 2050, human population is expected to reach 9.7 billion. The demand for increased food production needs to be met from ever reducing resources of land, water and other environmental constraints. Rice remains the staple food source for a majority of the global populations, but especially in Asia where ninety percent of rice is grown and consumed. Climate change continues to impose abiotic and biotic stresses that curtail rice quality and yields. Researchers have been challenged to provide innovative solutions to maintain, or even increase, rice production. Amongst them, the ‘green super rice’ breeding strategy has been successful for leading the development and release of multiple abiotic and biotic stress tolerant rice varieties. Recent advances in plant molecular biology and biotechnologies have led to the identification of stress responsive genes and signaling pathways, which open up new paradigms to augment rice productivity. Accordingly, transcription factors, protein kinases and enzymes for generating protective metabolites and proteins all contribute to an intricate network of events that guard and maintain cellular integrity. In addition, various quantitative trait loci associated with elevated stress tolerance have been cloned, resulting in the detection of novel genes for biotic and abiotic stress resistance. Mechanistic understanding of the genetic basis of traits, such as N and P use, is allowing rice researchers to engineer nutrient-efficient rice varieties, which would result in higher yields with lower inputs. Likewise, the research in micronutrients biosynthesis opens doors to genetic engineering of metabolic pathways to enhance micronutrients production. With third generation sequencing techniques on the horizon, exciting progress can be expected to vastly improve molecular markers for gene-trait associations forecast with increasing accuracy. This book emphasizes on the areas of rice science that attempt to overcome the foremost limitations in rice production. Our intention is to highlight research advances in the fields of physiology, molecular breeding and genetics, with a special focus on increasing productivity, improving biotic and abiotic stress tolerance and nutritional quality of rice.

Physiological, Molecular Breeding and Genetic Perspectives

Rice Improvement

Muhammad Tehseen Azhar

Wild Germplasm for Genetic Improvement in Crop Plants addresses the need for an integrated reference on a wide variety of crop plants, facilitating comparison and contrast, as well as providing relevant relationships for future research and development. The book presents the genetic and natural history value of wild relatives, covers what wild relatives exist, explores the existing knowledge regarding specific relatives and the research surrounding them and identifies knowledge gaps. As understanding the role of crop wild relatives in plant breeding expands the genetic pool for abiotic and biotic stress resistance, this is an ideal reference on this important topic. Provides a single-volume resource to important crops for accessible comparison and research Explores both conventional and molecular approaches to breeding for targeted traits and allows for expanded genetic variability Guides the development of hybrids for germplasm with increased tolerance to biotic and abiotic stresses

Wild Germplasm for Genetic Improvement in Crop Plants

Abiotic stress adversely affects crop production worldwide, decreasing average yields for most of the crops to 50%. Among various abiotic stresses affecting agricultural production, drought stress is considered to be the main source of yield reduction around the globe. Due to an increasing world population, drought stress will lead to a serious food shortage by 2050. The situation may become worse due to predicated global climate change that may multiply the frequency and duration and severity of such abiotic stresses. Hence, there is an urgent need to improve our understanding on complex mechanisms of drought stress tolerance and to develop modern varieties that are more resilient to drought stress. Identification of the potential novel genes responsible for drought tolerance in crop plants will contribute to understanding the molecular mechanism of crop responses to drought stress. The discovery of novel genes, the analysis of their expression patterns in response to drought stress, and the determination of their potential functions in drought stress adaptation will provide the basis of effective engineering strategies to enhance crop drought stress tolerance. Although the in-depth water stress tolerance mechanisms is still unclear, it can be to some extent explained on the basis of ion homeostasis mediated by stress adaptation effectors, toxic radical scavenging, osmolyte biosynthesis, water transport, and long distance signaling response coordination. Importantly, complete elucidation of the physiological, biochemical, and molecular mechanisms for drought stress, perception, transduction, and tolerance is still a challenge to the plant biologists. The findings presented in volume 1 call attention to the physiological and biochemical modalities of drought stress that influence crop productivity, whereas volume 2 summarizes our current understanding on the molecular and genetic mechanisms of drought stress resistance in plants.

Physiology and Biochemistry

Drought Stress Tolerance in Plants, Vol 1

Plant improvement has shifted its focus from yield, quality and disease resistance to factors that will enhance commercial export, such as early maturity, shelf life and better processing quality. Conventional plant breeding methods aiming at the improvement of a self-pollinating crop, such as wheat, usually take 10-12 years to develop and release of the new variety. During the past 10 years, significant advances have been made and accelerated methods have been developed for precision breeding and early release of crop varieties. This edited volume summarizes concepts dealing with germplasm enhancement and development of improved varieties based on innovative methodologies that include doubled haploidy, marker assisted selection, marker assisted background selection, genetic mapping, genomic selection, high-throughput genotyping, high-throughput phenotyping, mutation breeding, reverse breeding, transgenic breeding, shuttle breeding, speed breeding, low cost high-throughput field phenotyping, etc. It is an important reference with special focus on accelerated development of improved crop varieties.

Vegetable Crops

Accelerated Plant Breeding, Volume 2

Vinay Kumar

Penna Suprasanna

Soil salinity is a key abiotic-stress and poses serious threats to crop yields and quality of produce. Owing to the underlying complexity, conventional breeding programs have met with limited success. Even genetic engineering approaches, via transferring/overexpressing a single ‘direct action gene’ per event did not yield optimal results. Nevertheless, the biotechnological advents in last decade coupled with the availability of genomic sequences of major crops and model plants have opened new vistas for understanding salinity-responses and improving salinity tolerance in important glycophytic crops. Our goal is to summarize these findings for those who wish to understand and target the molecular mechanisms for producing salt-tolerant and high-yielding crops. Through this 2-volume book series, we critically assess the potential venues for imparting salt stress tolerance to major crops in the post-genomic era. Accordingly, perspectives on improving crop salinity tolerance by targeting the sensory, ion-transport and signaling mechanisms are presented here in volume 1. Volume 2 will focus on the potency of post-genomic era tools that include RNAi, genomic intervention, genome editing and systems biology approaches for producing salt tolerant crops.

Targeting Sensory, Transport and Signaling Mechanisms

Salinity Responses and Tolerance in Plants, Volume 1

Plant improvement has shifted its focus from yield, quality and disease resistance to factors that will enhance commerical export, such as early maturity, shelf life and better processing quality. Conventional plant breeding methods aiming at the improvement of a self-pollinating crop, such as wheat, usually take 10-12 years to develop and release of the new variety. During the past 10 years, significant advances have been made and accelerated methods have been developed for precision breeding and early release of crop varieties. This work summarizes concepts dealing with germplasm enhancement and development of improved varieties based on innovative methodologies that include doubled haploidy, marker assisted selection, marker assisted background selection, genetic mapping, genomic selection, high-throughput genotyping, high-throughput phenotyping, mutation breeding, reverse breeding, transgenic breeding, shuttle breeding, speed breeding, low cost high-throughput field phenotyping, etc. It is an important reference with special focus on accelerated development of improved crop varieties.

Shabir Hussain Wani

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