This book constitutes the refereed proceedings of the Second International Conference on Applied Intelligence and Informatics, AII 2022, held in Reggio Calabria, Italy, during September 1–3, 2022. The 38 full papers included in this book were carefully reviewed and selected from 108 submissions. They were organized in topical sections as follows: Emerging Applications of AI and Informatics; Application of AI and Informatics in Healthcare; Application of AI and Informatics in Pattern Recognition; and Application of AI and Informatics in Network, Security, and Analytics.