M. Sabu

Tombin Thomas

P. E. Sreejith 

 Prem Jose Vazhacharickal

Sajeshkumar N. K 

 Jiby John Mathew

Amylases are well known for applications ranging from starch and food processes industry to medical applications. The increased demand for these enzymes in various industries has led to an enormous interest in developing enzymes with better properties such as raw starch degrading amylases. It is suggested that banana peel and male inflorescence could employ as a promising substrate for the production of amylase by Aspergillus niger. Further, solid state fermentation is a better choice for amylase production. The addition of external growth medium is also found beneficial for increasing enzyme production. The present study was undertaken to isolate, identify and characterize the Aspergillus niger in the culture medium followed by amylase production and extraction. The banana parts used here as substrates are ripe fruit peel and male inflorescence from locally cultivated species Ethan (Nendran), Palayamkodan (Palayanthodan), Rasakadali (Njali Poovan) and Sundari. The result shows that amylase from sundari peel have the best activity followed by Ethan peel. Ethan flower bud shows the least activity among the eight substrates under study.

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