Prem Jose Vazhacharickal

Noby Mathew

Lakshmi B Nair

A simple and reproducible protocol for in vitro callus induction from explants of three endemic plants (Cyclea peltata, Naregamia alata and Kaempferia galanga Linn.) have been developed. Explants collected from the field grown plants were cultured on MS medium supplemented with different concentration/combination (s) of phytohormones. During the study period we evaluated the effect of different growth regulators in callus induction and its morphological analysis of the targeted plants. To optimize the callus induction of three different targeted explants were cultured on different concentration phytohormones. Among which the system include 2,4-D has the most efficient effect on the three experimental plants, Five different concentration taken for three explants, among that Cyclea peltata and Kaempferia galangal Linn. has the highest potential to induce callusing at 2 mg/L of 2,4 -D. In this study we found that there was no effect on callusing of the targeted plants was MS medium containing combination of auxin and cytokinin for callusing.

Efficient in vitro callus induction protocol for three endemic medicinal plants (Cyclea peltata, Naegamia alata and Kaempferia galangal Linn.) in Kerala

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